Current combination antiretroviral therapies (cART) efficiently suppress HIV-1 reproduction in humans, but the virus persists as integrated proviral reservoirs in small numbers of cells. To generate an antiviral agent capable of eradicating the provirus from infected cells, we employed 145 cycles of substrate-linked directed evolution to evolve a recombinase (Brec1) that site-specifically recognizes a 34-bp sequence present in the long terminal repeats (LTRs) of the majority of the clinically relevant HIV-1 strains and subtypes. Brec1 efficiently, precisely and safely removes the integrated provirus from infected cells and is efficacious on clinical HIV-1 isolates in vitro and in vivo, including in mice humanized with patient-derived cells. Our data suggest that Brec1 has potential for clinical application as a curative HIV-1 therapy.
Clinical trials are expected to start in Hamburg, Germany, soon.
Tips site How-To Geek discovered that Windows Spotlight—which normally rotates between a selection of photographs—was being used to display an ad for Square Enix’s Rise of the Tomb Raider. Understandably, most people probably don’t want to be hit in the face with a full-screen ad for a video game before they even unlock their computer. If you want to make sure you’re not hit with these ads, follow these steps to disable Windows Spotlight:
Open the Start Menu and search for “Lock Screen Settings.”
Under “Background,” select either Picture or Slideshow, instead of Windows Spotlight.
Scroll down to “Get fun facts, tips, tricks, and more on your lock screen” and this toggle.
Apparently the “and more” is where Microsoft hid the advertisements. (emphasis mine)
An additional 220,000 potential victims had information stolen from an IRS website as part of a sophisticated scheme to use stolen identities to claim fraudulent tax refunds, the IRS said Monday. The revelation more than doubles the total number of potential victims, to 334,000.
Well, no matter, this hack is mere chicken feed compared to the 21 million records stolen from the federal Office of Personal Management. And it hardly compares with the recent Pentagon breach, where the Chinese got almost all federal records. No, the IRS is doing a much better job then those other agencies, only being slightly incompetent and screwing up only a little.
I have never seen anything fill up a vacuum so fast and still suck. -- Rob Pike, on X.