chloroquine's Journal: Again with the elutions

It is Saturday evening and I'm sitting in lab. I've been here since about ten this morning and I'm trying to decide if I want to just grab my backpack and go home now while the going is good.

Since I last wrote in here, I've been working on the same project and making limited headway. It is easy to do protocols that I've done before and that are done regularly in the lab. Now I am faced with learning new techniques and progress has slowed.

My second project is still going strong. And hopefully I'll have some concrete data in the next few weeks. There are some technical details to iron out, but I'm convinced that they are not going to be too bad to deal with. It makes me nervous when I'm optimistic. I'm not naturally an optimist, so there must be something wrong. Perhaps inhaling a little too much ethanol in the tissue culture room.

I just read Frank Miller's book, Ronin for the first time a few days ago. I think I'm going to sit down and read it again tonight, because I feel like it has enough layers that I'm missing a whole slew of stuff in it. This isn't how I generally feel about comic books (graphic novels, whatever you want to call them).

Reading things on this site I'm reminded frequently how bloody ignorant I am of things computer. I wish for a translator or a guide to explain things to me. Then again, I already have enough to worry about with the dreaded pile of journal articles on my desk.

No need to feel ignorant.

[datastew]

Ask your questions here and helpful slashdot readers are always willing to point you in the direction of some answers.

Fellow Genejockey

[TitaniumFox]

Kudos on your commitment to scientific research. Like you, I'm working on protein:protein interactions, but mostely centered in BCL-2 family mediated apoptosis. Our lab does most of the interaction workup in yeast via 2-hybrid systems and then we move to transgenic mouse models.

I'm trying to recapitulate one of our classic knockout models using RNAi to validate it in our lab. It's pretty interesting stuff.

Re:Fellow Genejockey

[chloroquine]

I'm going to start on my first thing with RNAi probably on Tuesday if Dharmacon gets it shipped on Monday. Right now I'm learning all about the joys of metabolically labelling with tritium. Definitely no mouth pipetting there.

Just finished isolating a complex that turned out to be published in May by another lab. sigh. But at least that's something I can put aside so I can focus on the other fun project. We don't do much 2-hybrid stuff here because the background of the lab is purification of endogeno