One thing to note is, is that people are lazy and they easily forget they've subscribed to lists. I don't know what kind of list you have, but i would advise you to put very noticeable unsubscribe at the start of the list instead at the end of it. IE: i get some mails that i know i have subscribed to, but aren't what they were supposed to be. It would be easy to mark them as spam (people react emotionally to disappointment) especially since they hide unsubscribe options with the smallest font possible at the end of the message.
So my suggestion would be: make it easy for people to do the right thing and they'll do it. If you make it difficult people are very resourceful at doing the unexpected.
Another thing to be noted is that people were "trained" to not click unsubscribe option ("don't click unsubscribe or you'll just confirm your mail address as active to spammers" advice we've heard many times).
Again, I don't know what kind of list parent runs, these are just general observations from the lists I encountered.