This is a problem only if you don't know the field. Ultra-low temperature (-86c) freezers are a staple in any biological science lab around the world. -86c freezers are abundant, far from being high tech, not that expensive, and sold by a whole slew of companies.

The quality issue is not with the Real-Time PCR per say, the issue with is the RNA extraction. COVID-19 is a retrovirus, meaning it exists as RNA encapsulated in a protective cocoon (somewhat simplifying here). RNA is finicky and degrades quickly. RNA is single-stranded. PCR works with DNA. Good quality samples must be collected, samples are shipped to a lab and queued for processing (RNA degrades during that time), reasonable RNA quality must then be extracted (kits are used, those are the bottleneck with you hear kits are in short supply), but even with kits, on the lab side, there is a certain skill required to successfully extract RNA (the 'art of science' some would say). The samples are then used as template in a Real-Time PCR instrument to make enough copies so that we can detect a presence, or not. The first step of the Real-Time PCR is a Reverse Transcriptase cycle where RNA being converted to cDNA. RT-PCR (also called qPCR to avoid confusion with Reverse Transcriptase, q standing for quantitative) is fairly accurate and used broadly in a large array of clinical tests, there is a very solid track record there.

Collaborative Translation Framework

Details.... First sentence starts with 'in water'. Presumably, the next sentence is not 'in water'.

I am a Canadian expat in the Middle East who went through the trouble of hiring an experienced international tax lawyer from a major law firm to make sure our last tax return, and separation from Canada, was bulletproof. I hate to be the bearer of bad news, but being considered a 'Canadian tax resident' is not linked at all with physical residency. Being deemed a tax resident is linked with having enough ties back to Canada or demonstrating the intent of coming back. Spending more than half a year in Canada (this also dictates provincial taxes, which is all or nothing: a full year or none) certainly ticks both. However, even if you spend an entire fiscal year abroad, CRA can still deem you a tax resident owing the exact same amount of taxes to CRA as if you had worked in Canada (minus the difference paid in your country of residence, which in my case is el zippo). To add insult to injury, there is no actual expat tax code to follow, you can only go with prior court cases. But say you kept your Canadian professional membership, rented your house to your sister at below fair market value, kept all your bank and credit accounts, and contributed to your registered retirement plan: you will be deemed tax resident by CRA, no doubt about it.

Yup.

How is that trolling ? Title implies Dark Matter as a fact. Show me the proof for Dark Matter.

Wouldn't finding evidence of Dark Matter be the real news in this article ? Save from all the conjectures of course...

3 iPads, one of the two iPad 2s bricked on u/g, had to recover it via iTunes. The iPad air (Wifi, 128 Gb) has turned indeed into a pig. Performance is so poor, I couldn't even Skype with my parents this morning, Has Apple turned into MSoft ?

Magnetic beads have been used for decades to extract DNA from samples like Blood for instance. In that case we also use polarity: as DNA it negatively charged, it will bind to positively charged (/coated) bead allowing us to bind and wash the DNA then we can release the beads using low salts when the time for elution comes. An alternative to magnetic beads is membrane based purification but that involves a vacuum source and some form of contraption to force the 'liquid' though the membrane. In this case the beads are coated with a protein (MBL) that will bind (/capture) to the 'toxins'. The coating is what gives the specificity. Now the fact that the beads are magnetic is used to control the beads, capture them and release them at the appropriate stage at the process. In itself the magnetic property of the beads does nothing at the molecular biology level. Therefore the title "Artificial Spleen Removes .... Using Magnets" is completely misleading. But typical in my experience of how usually journalists understand nothing of the Science. I used to work for a company that made transgenic goats and boy oh boy we were always shocked at the news article after the visit, wondering where did the person picked up all this pile of nonsense that was printed. Makes me wonder what else I read is nonsense, for the very few times I was there and then read the article: you'd have to know the real story to find it in the article.

Who needs a dirty bomb when you have a tripe 7 as a missile. They could have landed in a number of countries such as North Korea, Myanmar, hell they could have almost made it to Pakistan. My money is on this being a prelude to something bigger.

This aircraft was introduced in 1966. In the context of the times, it makes no sense whatsoever that the team behind the plane just patted themselves on the back and moved on to other projects. A plane that as of today is still 'state of the art'. Simple logic dictates that there was a succesor and most likely by now, incremental iterations of said plane. The SR 71 was not retired because its mission was obsolete nor because of costs, but because the plane itself was obsolete.

> herpetology (the study of amphibians) err... Batrachology is the study of amphibians alone, Herpetology is the study of both amphibians and reptiles.

sorry for the massive typos,

Could it be that EC2's problems and PSN's are linked ? Have no clue myself, but seems to me PSN need large scalable bandwidth. Does anyone know where PSN is hosted ? How does it work says with COD ? Are COD servers on PSN or is PSN just a relay ?