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Comment Reproducibility (Score 2) 43

Whenever I see an article that reports that some laboratory experiment was not able to be reproduced, I always think back to my time as a graduate student in microbiology. We were growing L-form (cell wall deficient) Neisseria gonorrhea on media containing polyvinyl pyrrolidone. We found that some lots of PVP had an unknown inhibitor that we were not able to identify (nor able to dialyze out of the solution), and other lots worked fine. When we had a lot that worked, we'd call up Sigma Chemical, and order as much as we could of the working lot numbers. So, anyone trying to reproduce our results might be unsuccessful, unless they were using a 'good' batch of PVP.

Also, from that era, my thesis advisor told a story of a time when he was out having a beer with his advisor when he was doing his post-doc. His advisor commented that his research was insignificant, and he quipped back that his advisor's was irreproducible. His advisor didn't have a sense of humor, and essentially threw him out. His advisor was on the editorial board of the Journal of Bacteriology, which at the time was one of very few microbiology journals, and blackballed him from publishing in JBact for several years.

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