Furthermore, you don't need to kill the target for fluorescent microscopy. While attaching labels may affect some of the chemistry in the organism, you can at least still observe biological behavior.
Here's the relevant passage from the article with the juicy bits:
We acquired X-ray microscope images of these vitrified mammalian cells at tilt angles from 60 to +60 in increments of 1 at a pixel size of either 9.8 nm (25-nm zone plate objective) or 15.6 nm (40-nm zone plate objective). Exposure times for each tilt angle were 224 s. The total X-ray exposure (~109 Gy) produced negligible radiation damage, as we detected no difference in image quality between images acquired at the beginning and end of the tilt series (Supplementary Fig. 3). We processed the images using a reciprocal space algorithm11 to generate a 3D tomogram composed of cubic voxels whose side lengths were either 9.8 nm (25-nm zone plate objective) or 15.6 nm (40-nm zone plate objective).
So they took 121 x-ray images of the specimen, with each image taking 2-24 seconds, and then stitched them together using a tomography technique to obtain their 3D volume. It's certainly faster than a few weeks, but this is not what I would consider "immediate". The article also points out that poor cryopreservation led to some artifacts and that the resolution in this technique was still not as good as the TEM; not having an entire 180 degree rotation of the object led to artifacts as well:
We did not detect some structures by X-ray tomography that we detected by TEM, such as ribosomes and the double membrane of the mitochondrial cristae. These probably fall below the current resolution limit (see below). An additional limitation was the restricted tilt angle range (±60) used in these experiments. This led to poorer resolution in the z dimension, as indicated by a distortion in the 3D shape of some organelles, which appeared more cylindrical in x-z views (Fig. 3b) as well as an inability to obtain face-on views of the nuclear pores (data not shown) or follow the complete circumference of the nuclear membrane (Supplementary Fig. 5b).
So, you're saying she sounds like capsule and perfume?
On a more serious note, a (paid) update to Hatsune Miku gives her 6 additional voicing styles. I find that one of them might be able to sound somewhat like a real person if programmed well. Megpoid, using the same Yamaha sound engine, sounds a lot more realistic than the other Vocaloid2 products, and also doesn't require as much tweaking.
It's okay, because she's already under-aged to begin with. (16)
And there's no need for autotune! (Okay, maybe some autotune is in order when Miku refuses to sing the way you're telling her to sing...)
You can think of Hatsune Miku as the artist, or you can think of her as an instrument and the voice manipulator as the actual performer.
The original concert took place on March 9th. This comes out to 3/9, which, if you creatively pronounce it in Japanese, becomes "mi ku". The concert seems to me to be more of a Sega thing than a Crypton event, since the models they used were originally from the production of the PS3 rhythm game. Sitting in the audience was basically the closest you could've gotten to a Sharon Apple experience (sans glitching advanced strike craft).
I'm guessing it got brought to light because Sega and the software creators put on a series of showings of the Blu-Ray concert footage recently in the US.
Since she's virtual, and the creators openly encourage fan-art... you can see where this is going...
The 39 Miku day concert was held in Zepp Tokyo, which according to Wikipedia, has a capacity of around 2000 seats. It's "merely" a concert hall, not a stadium, but I think that's pretty good for a voice synthesizer.
The debut album featuring Hatsune Miku by supercell managed to top out at #4 on Oricon's weekly rankings for music sales when it was released. Also, if you take a look at Joysound, a major karaoke vendor in Japan that added Vocaloid songs to their catalog, Vocaloid songs occupy 9 out of the top 15 spots in the current weekly rankings across *their entire catalog*, not just the anime-related stuff. It missed out of the top 3, with those going to two AKB48 songs and the Eva opening song. So yes, not more popular than AKB48, but not totally unknown either.
Casio already had a color calculator way back when I was in high school. The curriculum still revolved around the use of the TI-83, though, so people with anything else were pretty much on their own.
That would be "natto tan". Nato-tan would obviously be a cute little anime girl mascot for NATO.
I think what the parent poster was trying to say was that mixing down a song is an art that requires experience, not something that a large chunk of the potential audience would possess. Those kernel developers weren't just random people you got off the street; they were people who had at least had experience with computer programming and knew C very well.
I think one way you can get the original idea to fly is to create some sort of automated mixing software that can take into consideration standard rules of thumb used in the mixing and mastering process and then perhaps allow the user to tweak the sound using a wizard type interface, e.g. click a button that says "This sounds too tinny" if the music is sounding thin. It's not going to be the best mix, but it should suffice for the user listening, since the mix doesn't have to work on ALL possible sound systems, just the current one the user is using.
I seriously doubt the typical listener would have as much experience with audio as Moby. By the way, does Moby do his own mixdowns and mastering or does he leave it up to someone else?
Real Users never use the Help key.
