HiClaw is a multi-agent operating system that is open source and operates on the Matrix framework, allowing various AI agents to work together within Matrix rooms, where their activities are fully accessible to humans in real-time. The system features a Manager Agent that oversees multiple Worker Agents, efficiently breaking down complex tasks and facilitating simultaneous execution, which enhances the management of these intricate operations. Designed with a focus on enterprise-level security and collaborative capabilities, HiClaw utilizes the open Matrix instant messaging protocol, ensuring that all communications between agents are transparent, easily auditable, and fit for distributed systems and federated environments. Humans have the ability to join any Matrix room whenever they wish, which allows them to monitor agent discussions, intervene as necessary, or adjust agent actions in real-time, thereby safeguarding oversight and control. This structured two-tier system, consisting of Manager and Worker Agents, delineates clear responsibilities for each agent, simplifying the process of integrating custom Worker Agents tailored for various applications, while also promoting adaptability within the architecture. Consequently, the design of HiClaw not only enhances operational efficiency but also paves the way for innovative uses of AI collaboration across diverse scenarios.