The secondary antibody should be used the same as with the use of an anti species sources, for example: If the one antibody is a monoclonal antibody of mouse origin, the secondary antibody is selected anti mouse secondary antibody, goat anti mouse or rabbit anti mouse etc. can be. If an antibody is prepared from rabbit serum, rabbit polyclonal antibody, the corresponding need to select the secondary antibodies anti rabbit secondary antibodies. That is, according to the source of a resistant species to select the appropriate secondary antibody against the species.
The secondary antibody need to match the categories or subcategories with an anti species. This is typically for the monoclonal antibody. The polyclonal antibody is an IgG class of immunoglobulin, and accordingly, the secondary antibody is anti IgG antibody. Classes and subclasses of monoclonal antibodies usually in the product manual, there will be described. If you have an anti mouse IgM, then the corresponding secondary antibody should be an anti mouse IgM. If monoclonal anti particular subclass of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) so almost all of the anti mouse IgG can be combined therewith. Or you can also choose specific subclasses secondary antibody. For example: If your anti mouse IgG1, then you can choose anti IgG1 antibody, this antibody is particularly suitable for double labeling experiments. Not clear an anti why species/sub class case, you can choose the appropriate species anti IgG.
The two antibodies to the choice of which probe is mainly depending on the experiment. For Western Blot and ELISA. The most commonly used secondary antibodies labeled secondary anti labeling experiments, cells or tissues Immunocytochemistry organization immunochemistry, flow cytometry, commonly used in the fluorophore labeled secondary antibody. Can also be used in immunohistochemistry to horseradish peroxidase or alkaline phosphatase labeled secondary antibody. If you want a greater degree of amplification of the detection signal, you can use the Biotin / Avidin detection system. Fluorescence detection programs, you need to choose a different fluorescently labeled gold particles conjugated secondary antibody used in immuno-electron microscopy.
Rabbit anti-human acyl-Coenzyme A oxidase 2, branched chain polyclonal Antibody"