Journal chloroquine's Journal: Again with the elutions 3
Since I last wrote in here, I've been working on the same project and making limited headway. It is easy to do protocols that I've done before and that are done regularly in the lab. Now I am faced with learning new techniques and progress has slowed.
My second project is still going strong. And hopefully I'll have some concrete data in the next few weeks. There are some technical details to iron out, but I'm convinced that they are not going to be too bad to deal with. It makes me nervous when I'm optimistic. I'm not naturally an optimist, so there must be something wrong. Perhaps inhaling a little too much ethanol in the tissue culture room.
I just read Frank Miller's book, Ronin for the first time a few days ago. I think I'm going to sit down and read it again tonight, because I feel like it has enough layers that I'm missing a whole slew of stuff in it. This isn't how I generally feel about comic books (graphic novels, whatever you want to call them).
Reading things on this site I'm reminded frequently how bloody ignorant I am of things computer. I wish for a translator or a guide to explain things to me. Then again, I already have enough to worry about with the dreaded pile of journal articles on my desk.
No need to feel ignorant. (Score:1)
Fellow Genejockey (Score:2)
I'm trying to recapitulate one of our classic knockout models using RNAi to validate it in our lab. It's pretty interesting stuff.
Re:Fellow Genejockey (Score:1)
Just finished isolating a complex that turned out to be published in May by another lab. sigh. But at least that's something I can put aside so I can focus on the other fun project. We don't do much 2-hybrid stuff here because the background of the lab is purification of endogeno