Comment Re:Old technique (Score 4, Informative) 58
"using partially coherent object illumination instead of previously used quasi-incoherent illumination"
which led to :
"We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ~36-nm (Rayleigh) and ~70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions."