I'm in the process of building a multiphoton microscope (donated axiovert 135 body and Hamamatsu detectors). Of course, I have nowhere near the amount of money to buy a Ti:Sapphire and would much rather spend the money on good IR lenses and upgraded optics.
I've been looking at "alternatives" in the form of Ytterbium based lasers for the reasons you describe: small 2 box footprint (with a pulse picker), integrated solid-state pump and no cavity tuning (or so the manufacturer says). Other advantage is the reduced photodamage with a two photon excitation around the 1030nm mark, but I am still waiting for an answer on the expected second harmonic / third harmonic signal generation efficiency (probably something I won't find out for certain until the entire microscope is built). 1-5% for THG seems frighteningly low, but fortunately, the detectors I'll be using have low dark count.
Also the 5nJ per pulse spec from the manufacturer worries me slightly. One thing I haven't quite worked out from the available literature is the discrepancy between the available energy (say 60-100nJ per pulse from a Yb:KGW laser) and the energy deposited on the sample (around 1nJ per pulse, when mentioned). I hope the loss comes from using a neutral density filter and not from losses due to the microscope optics...
With the right skills of course, the whole process of building your own microscope seems easy enough
I have never in my life seen or heard anyone use decimal points or fractions with Celsius degrees in an everyday context
You're missing out
One amateur reprocessed images from Soviet Venus landers and brought out some amazing detail, finding landscape features that weren't spotted before. It's simply the case that sometimes amateurs are simply motivated to spend the necessary time and attention to detail more so than "professionals", who normally have full in-boxes. Amateurs can decide to be as anal as they want. Call it open-source astronomy.
Thanks! I looked it up, and if you are referring to Don Mitchell's story, it is indeed well worth reading. http://www.mentallandscape.com/C_CatalogVenus.htm
Even better, the re-processing pipeline for each of the Venera mission datasets is explained in great detail. For instance, about the Venera-9 mission images (from http://www.mentallandscape.com/V_DigitalImages.htm:
The upper image is the raw 6-bit telemetry, about 115 by 512 pixels. Automatic gain control and logarithmic quantization were used to handle the unknown dynamic range of illumination. Previously published images from these probes suffered from severe analog generation loss, so it is fortunate that the original data was found. The raw image was converted to optical density according to Russian calibration data, then to linear radiance for image processing. It was interpolated with windowed sinc filter to avoid post-aliasing (a "pixilated" appearance), and the modulation transfer function ("aperture") of the camera was corrected with a 1 + 0.2*frequency**2 emphasis. This was then written out as 8-bit gamma-corrected values, using the sRGB standard gamma of 2.2. Some of the telemetry bars on the right were replaced with data from the 124 panorama. The bottom image is digitally in-painted, using Bertalmio's isophote-flow algorithm, to fill in missing data.
The measurement results showed that the circuit performs as expected and it achieves a CMRR of 82 dB and a maximum SNDR of 36.1 dB with 1-V supply voltage. The total current consumption of the chip, including the output buffer, is only 3 A. Furthermore, the average current consumption of the 8th order bandpass SO-SC filter is very low, since it consumes only about 400 nA, corresponding to 50 nA/pole, which is much less than the current consumption of the SC filters in prior designs. Therefore, the circuit is very suitable for portable ECG measurement applications, like heart rate detectors.
Not sure if anything like this is available commercially though... and of course, using off-the-shelf electronics will cut the costs right down.
SPAD afterpulsing is probably not an issue for this project because it's looking at photon pairs, so uncorrelated random events occurring on all the SPADs won't affect the detection... but will decrease the counting/processing rate by bogging down the electronics.
For measuring concurrent events, I would've thought TTS would be much more critical, and you can't get much better than MCP-PMTs (10-20ps these days?). Just wondering if the same detection could've been done with a multi-anode MCP, although if the sensor is CMOS tech, APDs would probably be easier to incorporate onto an ASIC / SoC.
When did FTP become the "web"?
About the same time gopher was being phased out?
Yes, if you want to be pedantic, I meant URL, not web address (since you so cleverly pointed at the ftp/ftps schemes). Here's my geek card, I give up...
This what I ended-up using:
((?:http|ftp)s?://)?(((([\d]+\.)+){3}[\d]+(/[\w./]+)?)|([a-z]\w*((\.\w+)+){2,})([/][\w.~]*)*)
There may well be something more robust...
Stellar rays prove fibbing never pays. Embezzlement is another matter.