1) PDF version http://devbio.wustl.edu/InfoSo...
2) Commentary, 2004: http://www.jbc.org/content/280...
3) Wikipedia: http://en.wikipedia.org/wiki/L...

"The Lowry protein assay is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is exhibited by a color change of the sample solution in proportion to protein concentration, which can then be measured using colorimetric techniques. It is named for the biochemist Oliver H. Lowry who developed the reagent in the 1940s. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature, cited over 200,000 times."

The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is best used with protein concentrations of 0.01–1.0 mg/mL and is based on the reaction of Cu+, produced by the oxidation of peptide bonds, with Folin–Ciocalteu reagent (a mixture of phosphotungstic acid and phosphomolybdic acid in the Folin–Ciocalteu reaction). The reaction mechanism is not well understood, but involves reduction of the Folin–Ciocalteu reagent and oxidation of aromatic residues (mainly tryptophan, also tyrosine). Experiments have shown that cysteine is also reactive to the reagent. Therefore, cysteine residues in protein probably also contribute to the absorbance seen in the Lowry Assay. [3] The concentration of the reduced Folin reagent is measured by absorbance at 750 nm.[4] As a result, the total concentration of protein in the sample can be deduced from the concentration of Trp and Tyr residues that reduce the Folin–Ciocalteu reagent.

The method was first proposed by Lowry in 1951. The Bicinchoninic acid assay and the Hartree–Lowry assay are subsequent modifications of the original Lowry procedure.

Which is what this boils down to.

There aren't any mysteries here at all.

Then problem goes away!

And the funny thing is, super-complicated password are an anti-security measure.

Because if the password is hard to remember, chances are the user has it written on a piece of paper or a note somewhere..

Normal passwords don't tend to suffer from this problem.

If the super-complex password requires causes the user to write it down or store it on their phone or such, it is hurting security --- not helping it.

Good points. It is has been so long I didn't recall it wasn't Boot Camp pre-Intel and it was slow.

I can't imagine a more vapid and informationless article with no context of history.

That bar graph of a spike starting in 2007 would more likely be related to the release of the iPhone.

Developing for the iPhone required a Mac. That was Apple's "killer app" for the Mac. Anyone wanting to get in to iPhone development had to have a Mac and it started mainstreaming the Mac.

Without the development of the iPhone it is hard to see an particular strong reason for Mac marketshare to start growing (sure you have the characteristic that it is very hard to get malware on a Mac, but that alone doesn't make up for the lack of apps or games, which was more far severe back in 2007 and not quite as bad today. The selection of software on a Mac is okish today, but in 2007 it was downright terrible.)

Pre-Intel (2007 +/-), the Mac did have Bootcamp (the ability to load and boot Windows on the machine) and software development was about the same before Intel and after, it isn't like casual developers are writing in assembly language --- the compiler (usually) takes care of all the fine details and endianess really only enters the equation when reading files with specific byte ordering of values.

Most industries depend on just-in-time processes to minimize inventory.

Timing is critical and tens of millions of dollars can be stake easily if it doesn't work like a clock.

There isn't going to be the tolerance for stranded trucks with flat tires that can't be repaired without a human, or late trucks stuck in traffic.

And changing lanes in a truck in a traffic jam? Haha, yeah. And making turns in a truck in a tight intersection? Sure. And trucks are tall, there are bridges and all kinds of height obstacles out there requiring special routes.

Road construction? A minor inconvenience for a car can be a major dilemma for a truck --- things like roads with no shoulders, etc.

And single truck wreck would carry large costs, and would insurance companies covering the transportation companies put up with it?

I think the idea is that people don't carry printers with them.

Anything requiring a printer is a bit broke by design in the modern age.

Really? The RIM lawsuit was over and done with 8+ years ago.

Blackberry doesn't exist today because they were about "enterprise solutions" and catered to big business and government.

Once the iPhone was released, no one wanted clunky 1990s style phones and Blackberry didn't have a plan. And when Blackberry finally had a plan, it was a stupid plan to make their OS instead of using Android.

Blackberry now gets to join Nokia in the club of phone companies no longer around because they didn't use Android.

The casual user will set this up and then log into Facebook and check their gmail. Totally secure!!! Absolutely anonymous!

Oh, and don't forget the cross-domain 'Flash cookies'!

You did an unintentional double entendre there. You know, like, the weak link is the "users" as in user failing to understand how to safely browser anonymously and inadvertently compromise their security using Tor. Or the weak link is the "users" as in 'although the idea of anonymity is great' and some casual users will be attracted, it will be a magnet for underground malfeasance and Silk Road wannabees and drug traffickers.

Goodbye ISIL, hello Crimson Jihad!

I know this chap means well, but in Middle East they won't be used for freedom.

They will be used to further even more violence and jihad.

Not dissimilar to how food shipments to poor countries doesn't go to hungry people, but the thugs with guns. Or how medical workers in Liberia get attacked.

The world doesn't quite work how we would like it to work. Sadly.

In Africa, they don't have proper sanitation.

Feces is a top spreader of disease. In much of the world, we are not using outhouses or the like.

> Media's doing what media does, hyping and scaring to rake in eyeballs and sell their advertisements.

Which is kind of a shame. The media, needing dollars, likes to capitalize on sensationalism.

At the same time, the reality is all news may be a form of entertainment, directly or indirectly. The news is related to the random "interesting topic du jour".

I used to do the 'drive a car forever thing'. Now, I just want reliability and no worries. Driving a car for 15 years will always be the most economical.